Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Morphology of BT20 (I.), MCF7 (II.) and MCF10A (III.) cell lines propagating as a monolayer on TCP (AC) and spheroids on PDMS (D–F) 48h after seeding [seeding density=50,000 cells/well of a 24-well plate, scale bars=200 μm]. MTT assay for determining the metabolic activity of cells indicate that there is no statistically significant difference in the metabolic activity of cells on PDMS and TCP (G).

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: MTT Assay, Activity Assay

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Scatter plots from flow cytometry experiments indicating the percentage of cells binding to soluble E-selectin in BT20 (A and D), MCF7 (B and E) and MCF10A (C and F) in monolayer (A–C) and spheroid (D–F) morphology.

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Flow Cytometry, Binding Assay

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Confocal microscopy images of co-culture spheroids with BT20 (blue) MCF7 (green) and MCF10A (red) cells on PDMS (A) by the end of 48h. Individual fields showing BT20 (B), MCF7 (C) and MCF10A (D) cells. [Scale bars=50 μm]

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Confocal Microscopy, Co-Culture Assay

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Confocal microscopy images of soluble E-selectin (orange) binding to whole co-culture spheroids with BT20 (blue) MCF7 (green) and MCF10A (red) cells on PDMS (A). Superimposed images of soluble E-selectin with individual cell types in co-culture indicate preferential binding of soluble E-selectin to BT20 cells in co-culture (B) when compared to MCF7 (C) or MCF10A cells (D) in co-culture. [Scale bars=50 μm]

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Confocal Microscopy, Binding Assay, Co-Culture Assay

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Flow cytometry histograms indicate a shift in fluorescent intensity peak for soluble E-selectin binding in monolayer, spheroid, monolayer co-culture and spheroid co-culture with respect to isotype control for BT20 (A), MCF7 (B) and MCF10A (C) cells.

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Flow Cytometry, Binding Assay, Co-Culture Assay, Control

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Fluorescent images showing BT20 (blue) MCF7 (green) and MCF10A (red) cells from co-culture spheroids invading the Matrigel (A). Individual fields indicate that the number of BT20 cells invading the matrigel (B) is higher than the number of MCF7 (C) or MCF10A (D) cells invading the Matrigel-coated transwell.

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Co-Culture Assay

Journal: Biomaterials

Article Title: Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

doi: 10.1016/j.biomaterials.2012.08.052

Figure Lengend Snippet: Western blot membranes showing the expression of HIF-1α and HIF-1β in spheroid, monolayer (Normoxia) and monolayer cells treated with cobalt chloride (Hypoxia) in BT20 (A and B), MCF7 (C and D) and co-culture spheroids (E and F). The ratio indicated in the figure corresponds to BT20:MCF7:MCF10A cells in co-culture. The band intensity computed using ImageJ indicates that expression of HIF-1α increased in co-culture conditions (G).

Article Snippet: Breast cancer cell lines BT20 (ATCC, HTB-19) and MCF7 (ATCC, HTB-24) were cultured in ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30-2003) supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11050H), and 1% penicillin/streptomycin (PS) (Invitrogen, 15140-122).

Techniques: Western Blot, Expressing, Co-Culture Assay

Journal:

Article Title: External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator

doi: 10.1073/pnas.202162199

Figure Lengend Snippet: Effects of TAT-ESX129–145 on the protein levels of Her2 in Her2-expressing breast cancer cell lines. TAT-ESX129–145 (12.5 μM) significantly reduced the protein levels of Her2 in all of the three cell lines tested (SK-BR-3, MDA-MB-453, and MCF7), whereas TAT alone or TAT-VP16469–485 had little effects. The loaded amounts of cell lysates were normalized by Bradford assays and Coomassie blue staining. The Her2 protein and β-actin were detected by Western blots.

Article Snippet: HeLa S3 and MCF7 cells were purchased from American Type Culture Collection.

Techniques: Expressing, Staining, Western Blot

Journal:

Article Title: External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator

doi: 10.1073/pnas.202162199

Figure Lengend Snippet: (A and D) Effects of TAT-ESX129–145 on the growth of breast cancer cells. Five breast cancer cell lines were incubated with TAT-ESX129–145. TAT-ESX129–145 impaired the growth and viability of Her2-expressing breast cancer cells (SK-BR-3, MDA-MB-453, and MCF7), whereas it had much less effect on MDA-MB-468 cells with no detectable levels of Her2. MCF7-HER18, an MCF7-derived cell line in which Her2 is overexpressed on a heterologous promoter, was resistant to TAT-ESX129–145. The concentration of TAT-ESX129–145 is 25 μM. (B) Nuclear condensation induced by TAT-ESX129–145. SK-BR-3 and MDA-MB-468 cells were incubated with TAT-ESX129–145 (25 μM) or TAT (25 μM) for 8 h. The cells were then fixed and stained with Hoechst 33342 (10 μg/ml) for 10 min. A typical apoptotic phenotype of the nuclei was observed only when the Her2-overexpressing SK-BR-3 cells were incubated with TAT-ESX129–145. (C) Western blots of Her2. It is evident that MDA-MB-468 has no detectable levels of Her2.

Article Snippet: HeLa S3 and MCF7 cells were purchased from American Type Culture Collection.

Techniques: Incubation, Expressing, Derivative Assay, Concentration Assay, Staining, Western Blot